Proteomics Strand
Project 2: Biotin-tagged Nuclear Receptors and Coregulators for Proteomic and Genomic Analysis
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Christopher K. Glass
Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA
Ph.D & M.D.: 1984, UCSD
ckg@ucsd.edu |
Specific Aims
1. Generate expression vectors for biotin-tagging of all mouse and human nuclear receptors, and a subset of human and mouse coregulators.
2. Generate mouse macrophage cell lines that express biotin-tagged versions of the NRs found to be normally expressed in macrophages, based on the NURSA expression dataset.
3. Perform ChIP-ChIP analysis of a subset of biotin-tagged nuclear receptors and coregulators in stable cell lines to define their genomic locations on a large scale.
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