Proteomics Strand

Project 1: Generation of ES Cells for Tissue-Specific Overexpression of Nuclear Receptors and Coregulators in vivo

Ming-Jer Tsai	Sophia Y. Tsai	Francesco J. DeMayo
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX	Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX	Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX
Ph.D: 1969, University of California, Davis	Ph.D: 1969, University of California, Davis	Ph.D: 1983, Michigan State University
mtsai@bcm.edu	stsai@bcm.edu	fdemayo@bcm.edu

Specific Aims

1. Construction of a conditional expression vector for easy insertion of the NRs and CoRs that can then be knocked into the ROSA26 locus by homologous recombination in ES cells

2. Generation of ES cells with the NRs and CoRs overexpression cassette inserted into the ROSA26 locus

3. Generation of mice over expressing one NR and one CoR each for proof of principle


Information on Ming-Jer Tsai's Phase I NURSA Project

Information on Sophia Tsai & Franco deMayo's Phase I NURSA Shared Resource