by Rainer B. Lanz, Ph.D.

An Introduction in Real-Time Quantitative PCR

Basics on methodolgy, protocols, assays, and ways of quantification
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Real-time QPCR on ABI's 7700, 7500 and StepOnePlus Systems

A tutorial on systems, operation, sequence detection software (SDS), and using the MCB Q-PCR facility
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Proper Amplicon Design for Real-Time QPCR

A tutorial on A) comprehensive target sequence analysis and B) amplicon design with Primer Express(®)
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QPCR Data Analyses and Evaluation

A tutorial on assay evaluation and troubleshooting sub-optimal Q-PCR experiments
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Curing QPCR Malignancies

Expert advice on streamlining Q-PCR experiments
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OpenHelix Quick Reference: UCSC

Quick Reference card for the UCSC Genome Browser
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A QPCR Tutorial for the PI

Questions a PI should ask when presented with Q-PCR data
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These tutorials are provided as an educational service by NURSA, the Nuclear Receptor Signaling Atlas, an NIH-funded researcb consortium on nuclear receptors and coregulators. Dr. Rainer Lanz is an Assistant Professor at Baylor College of Medicine and a member of the NURSA Bioinformatics Resource.